科學(xué)研究：

研究方向：

主要從事工業(yè)酶基因的選殖、酶蛋白純化、基因改造、酶學(xué)研究（包含熱力學(xué)及動(dòng)力學(xué)）、X光蛋白晶體學(xué)、膜蛋白三度空間結(jié)構(gòu)之解析、工業(yè)酶及膜蛋白發(fā)酵技術(shù)探究。

承擔(dān)科研項(xiàng)目情況：

1． 科技支撐計(jì)劃項(xiàng)目：新型紡織酶的X光結(jié)構(gòu)解析、基因改造及開發(fā)，負(fù)責(zé)人。

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科研成果：

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論文專著：

在 PNAS， JBC，Structure，JACS，JMB，Proteins 等雜志上發(fā)表 SCI 論文共20余篇。

出版專著：

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發(fā)表論文：

1. Guo, R. T., Chou, L. J., Chen, Y. C., Chen, C. Y., Pari, K, Jen, C. Y., Lo, S. C., Huang, S. L., Lee, C. Y., Chang, T. W., Chuang, W. J. (2001) Expression in Pichia pastoris and Characterization by Circular and NMR of Rhodostomin. Proteins: Structure, Function, and Genetics. 43, 499-508.

2. Guo, R. T., Ko, T. P., Chou, C. C., Shr, H. L., Chu, H. M., Tsai, Y. H., Liang, P. H., and Wang, A. H.-J. (2003) Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli. Acta Crystallogr. Sect. D Biol. Crystallogr. 59, 2265-2268.

3. Guo, R. T., Kuo, C. J., Chou, C. C., Ko, T. P., Shr, H. L., Liang, P. H., and Wang, A. H.-J. (2004) Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination. J. Biol. Chem. 279, 4903-4912.

4. Guo, R. T., Kuo, C. J., Ko, T. P., Chou, C. C., Liang, P. H., and Wang, A. H.-J. (2004) A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long-chain trans-prenyl products. Biochemistry. 43, 7678-7686.

5. Chu, H. M., Guo, R. T., Lin, T. W., Chou, C. C., Shr, H. L., Lai, H. L., Tang, T. Y., Cheng, K. J. Selinger, B. L., and Wang, A. H.-J. (2004) Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis. Structure. 12, 2015-2024.

6. Guo, R. T., Ko, T. P., Chen, A. P.-C. Kuo, C. J., Wang, A. H.-J., and Liang, P. H. (2005) Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate and farnesyl thiopyrophosphate: Roles of the metal ion and conserved residues in catalysis. J. Biol. Chem. 280, 20762-20774.

7. Sun, H. Y., Ko, T. P., Kuo, C. J., Guo, R. T., Chou, C. C., Liang, P. H., and Wang, A. H.-J. (2005) Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures. J. Bacteriol. 187, 8137-8148.

8. Chang, T. H., Guo, R. T., Ko, T. P., Wang, A. H.-J., and Liang, P. H. (2006) Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination. J. Biol. Chem. 281, 14991-15000.

9. Guo, R. T., Cao, R., Ko, T. P., Chang, T. H., Jeng, W. Y., Kuo, C. J., Chen, C. K.-M., Hudock, M. P., Zhang, Y., Song, Y., Liang, P. H., Oldfield, E., and Wang, A. H.-J. (2007) Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases. Proc. Natl. Acad. Sci. USA 104, 10022-10027.

10. Jeng, W. Y., Ko, T. P., Liu, C. I., Guo, R. T., Liu, C. L., Shr, H. L., and Wang, A. H.-J. (2008) Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis. Nucleic Acids Res. 36, 1567-77.

11. Kuo, C. J., Guo, R. T., Lu, I. L., Liu, H. G., Wu, S. Y., Ko, T. P., Wang, A. H.-J., and Liang, P. H. (2008) Structure-based inhibitors exhibit differential activities against Helicobacter pylori and Escherichia coli undecaprenyl pyrophosphate synthases. J Biomed Biotechnol. 2008, 841312.

12. Cao, R., Chen, C. K.-M., Guo, R. T., Wang, A. H.-J., and Oldfield, E. (2008) Structures of a potent phenylalkyl bisphosphonate inhibitor bound to farnesyl and geranylgeranyl diphosphate synthases. Proteins: Structure, Function, and Genetics. 73, 431-9.

13. Chen, C. K.-M., Hudock, M. P., Zhang, Y., Guo, R. T., Cao, R., No, J. H., Liang, P. H., Ko, T. P., Chang, T. H., Chang, S. C., Song Y. C., Axelson, J., Kumar, A., Wang, A. H.-J., and Oldfield, E. (2008) Inhibition of Geranylgeranyl Diphosphate Synthase by Bisphosphonates: A Crystallographic and Computational Investigation. Journal of Medicinal Chemistry. 51, 5594-607.

14. Wu, Y.H., Ko, T.P., Guo, R.T., Hu, S.M., Chuang, L.M., Wang, A.H.-J. (2008) Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2. Structure. 16, 1714-23.

15. Zhang, Y., Cao, R., Yin, F., Hudock, M., Guo, R.T., Krysiak, K., Mukherjee, S., Gao, Y.G., Robinson, H., Song, Y., No, J.H., Bergan, K., Leon, A., Cass, L., Goddard, A., Chang, T.K., Lin, F.Y., van, B.E., Papapoulos, S., Wang, A.H.-J, Kubo, T., Ochi, M., Mukkamala, D., Oldfield, E. (2009) Lipophilic Bisphosphonates as Dual Farnesyl/Geranylgeranyl Diphosphate Synthase Inhibitors: An X-ray and NMR Investigation . JACS 131, 5153-62.

16. Chen, C.K.-M., Lee, G.C., Ko, T.P., Guo, R.T., Huang, L.M., Liu, H.J., Ho, Y.F., Shaw, J.F., Wang, A.H.-J. (2009) Structure of the Alkalohyperthermophilic Archaeoglobus fulgidus Lipase Contains a Unique C-Terminal Domain Essential for Long Chain Substrate Binding. Journal of Molecular Biology. 390, 672-85.

17. Guo, R.T., Chong, Y.E., Guo, M., Yang, X.L. (2009) Unique mechanism for Ap4A synthesis by human Glycyl-tRNA synthetase. J. Biol. Chem. 284, 28968-76.

18. Cheng, Y.S., Ko, T.P., Wu, T.H., Ma, Y., Huang, C.H., Lai, H.L., Wang, A.H.-J., Liu, J.R.,* and Guo, R.T.* (2011) Crystal structure and substrate-binding mechanism of cellulase 12A from Thermotoga maritima. Proteins: Structure, Function, and Bioinformatics.79, 1193-204. (featured on cover)

19. Zeng, Y.F., Ko, T.P., Lai, H.L., Cheng, Y.S., Wu, T.H., Ma, Y., Chen, C.C., Yang, C.S., Cheng, K.J., Huang, C.H., Guo, R.T.* and Liu, J.R.* (2011) Crystal structures of Bacillus alkaline phytase in complex with calcium and inositol hexasulfate. Journal of Molecular Biology 409, 214-24. (featured on cover)

20. Wu, T.H., Huang, C.H., Ko, T.P., Lai, H.L., Ma, Y., Chen, C.C., Cheng, Y.S., Liu, J.R.,* and Guo, R.T.* (2011, in press) Diverse Substrates Recognition Mechanism Revealed by Thermotoga maritima Cel5A Structures in Complex with Cellobiose and Mannotriose. BBA-Proteins and Proteomics. (featured on cover)

21. Tseng, C.W., Ko, T.P., Guo, R.T., Huang, J.W., Wang, H.C., Huang, C.H., Cheng, Y.S., Wang, A.H.-J., and Liu, J.R. (2011, in press) Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata. Acta Crystallographic Section F. 67, 1189-1195. (featured on cover)

22. Huang, J.W., Cheng, Y.S., Ko, T.P., Lin, C.Y., Lai, H.L., Chen, C.C., Ma, Y., Zheng, Y., Huang, C.H., Zou, P., Liu, J.R.,* and Guo, R.T.* (2011, in press) Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-β-D-glucanase. Appl Microbiol Biotechnol.

榮譽(yù)獎(jiǎng)勵(lì)：

1． 2007年獲中國(guó)化學(xué)制藥股份有限公司第十七屆王民寧先生獎(jiǎng)之國(guó)內(nèi)醫(yī)藥研究所博士班優(yōu)秀論文獎(jiǎng)第一名。

2． 2007年獲臺(tái)大生命科學(xué)院博士畢業(yè)生院長(zhǎng)獎(jiǎng)第一名。

3． 2007年獲臺(tái)灣行政院國(guó)家科學(xué)委員會(huì)補(bǔ)助赴國(guó)外從事博士后研究之出國(guó)留學(xué)獎(jiǎng)學(xué)金。

4． 2010年入選天津千人計(jì)劃。

5． 2011年入選中國(guó)科學(xué)院“百人計(jì)劃”。

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